Journal: Science signaling

Article Title: Allosteric signaling through an mGlu2 and 5-HT2A heteromeric receptor complex and its potential contribution to schizophrenia.

doi: 10.1126/scisignal.aab0467

Figure Lengend Snippet: Fig. 5. Model for the docking of Gi/o onto the 5-HT2A–mGlu2 heteromeric receptor complex. (A to C) Ribbon representation of the vertical (A), extracellular (B), and cytoplasmic (C) views of the mechanism by which coupling of Gi/o proteins to the mGlu2 protomer located distal to the 5-HT2A component is necessary to enable allosteric crosstalk with 5-HT2A and consequently activate Gq/11- dependent signaling. The homodimeric interfaces of mGlu2 and 5-HT2A involve residues from TM1. The interface of the 5-HT2A–mGlu2 heteromeric complex involves residues from TM4. aAH, a-helical domain of the Ga subunit; aRas, Ras-like domain of the Ga subunit.

Article Snippet: For confirmation of specificity of the primary antibody (catalog no. RA24288; Neuromics) against 5-HT2A receptors in experiments with knockout mice, see fig. S9.

Techniques:

Journal: Journal of Microbiology and Biotechnology

Article Title: The Effect of Lactobacillus gasseri BNR17 on Postmenopausal Symptoms in Ovariectomized Rats

doi: 10.4014/jmb.2105.05032

Figure Lengend Snippet: Serum estradiol (E2) ( A ), Calcitonin ( B ), Osteocalcin ( C ), and 5-HT-2A ( D ) were measured after 14 weeks of initial administration ( n = 6~8 rats per group). Sophora japonica fruit extract (SJFE) treatment was used to create the positive control.

Article Snippet: The concentrations of estradiol (E2, CSB-E05110r), osteocalcin (CSB-E05129r), calcitonin (CSB-E05132r), estradiol (CSB-E05110r), and Serotonin 2A (5-HT-2A, CSB-E14956r) were assessed using an ELISA kit (CUSABIO, China).

Techniques: Positive Control

Journal: Biomolecules

Article Title: The Novel Pimavanserin Derivative ST-2300 with Histamine H 3 Receptor Affinity Shows Reduced 5-HT 2A Binding, but Maintains Antidepressant- and Anxiolytic-like Properties in Mice

doi: 10.3390/biom12050683

Figure Lengend Snippet: Binding affinity or percentage inhibition of ST-2300 or PIM at different GPCRs.

Article Snippet: HEK293 cells with a stable expression of human 5-HT 1A , 5-HT 6 , 5-HT 7b , and D 2L (prepared with the use of Lipofectamine 2000), or CHO-K1 cells with plasmids containing the sequence coding for the human serotonin 5-HT 2A receptor (PerkinElmer), were maintained at 37 °C in a humidified atmosphere with 5% CO 2 and grown in Dulbecco’s Modified Eagle Medium containing 10% dialyzed fetal bovine serum and 500 μg/mL G418 sulfate.

Techniques: Binding Assay, Inhibition

Journal: Ecotoxicology and environmental safety

Article Title: Chronic developmental exposure to low-dose ([C8mim][PF6]) induces neurotoxicity and behavioural abnormalities in rats.

doi: 10.1016/j.ecoenv.2021.112806

Figure Lengend Snippet: Fig. 4. Developmental exposure to low-dose ([C8MIM] [PF6]) increased the expression of NMDA receptors. (A) The mRNA level of major receptors (nmdar1, nmdar2a, nmdar2b for NMDA receptor; drd1 and drd2 for dopamine receptor; 5-ht2ar for 5-HT receptor; gabaarα1 for GABA receptor) for main neurotransmitters in hippocampus and prefrontal cortex. (B) The protein levels of NMDA receptors (NMDAR1, NMDAR2A, and NMDAR2B) in the hippocam pus and prefrontal cortex. (C) The protein levels of dopa mine receptors (DRD1 and DRD2), 5-HT receptors (5- HT2AR), and GABA receptors (GABAARα1) in the hippo campus and prefrontal cortex. (D) Statistical analysis of protein expression. n = 3 for each group. Student’s t-test was performed for the analysis. The data are presented as the mean ± SEM. *p＜0.05, **p＜0.01, ***p＜0.001.

Article Snippet: Membranes were treated with 5% skim milk for 1 h, followed by incubation with primary antibodies against NMDAR1 (ab109182, Abcam), NMDAR2A (ab124913, Abcam), NMDAR2B (ab65783, Abcam), DRD1 (ab81296, Abcam), DRD2 (ab30743, Abcam), 5-HT2AR (PB9599, Boster), GABAARα1 (ab252430, Abcam), PSD95 (36233S, Cell Signalling Technology), IBA1 (GTX100042, GeneTex), GFAP (BA0056, Boster), NeuN (ab177487, Abcam), MBP (BA0094, Boster), IFN-γ (PAA033Ra01, Cloud-clone Corp.), IL-1β (PAA563Ra01, Cloud-clone Corp.), TNF-α (PAA133Ra01, cloud-clone corp.), NF-ĸB (66535-1-lg, Proteintech), Histone H3 (AF0009, Beyotime), NRF2 (bs-1074R-1, bioss), PPARγ(ab272718, Abcam), COX2 (ab179800, Abcam), iNOS (ab178945, Abcam), Caspase 3 (9662s, CST), Bax (SC-7480, SANTA CRUZ), Bcl-2 (sc7382, SANTA CRUZ), GAPDH (5174T, CST), and β-actin (3700s, CST) at a 1:1000 dilution overnight at 4 ◦C.

Techniques: Expressing

Journal: Frontiers in Psychiatry

Article Title: Histone deacetylase inhibitors mitigate antipsychotic risperidone-induced motor side effects in aged mice and in a mouse model of Alzheimer’s disease

doi: 10.3389/fpsyt.2022.1020831

Figure Lengend Snippet: Effects of histone deacetylase (HDAC) inhibitors on histone acetylation at the 5htr2a and Drd2 gene promoters in the striatum in young and aged mice. (A) No significant differences of H3K27ac binding at the 5htr2a promoter between young and aged group was found, but a significant increase in H3K27ac binding at the 5ht2a promoter in the striatum was found in aged and young mice co-treated with RIS and VPA or MS-275 compared to those treated with RIS alone. (B) H3K27ac binding at the Drd2 promoter was significantly decreased in aged mice as compared to young mice in Veh treated group. However, H3K27ac binding at the Drd2 promoter in the striatum was observed to be significantly increased in aged mice co-treated with RIS and HDAC inhibitors VPA or MS-275 when compared to those treated with Veh alone. Data are presented as mean ± SEM, ( n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Blots were blocked for 1-hour with 5% non-fat dry milk (Biorad, 1706404) and incubated using 1:1,000 5HT2A (Boster Biological, PA1373), 1:500 D2R (Millipore, AB5084P), or 1:1000 B-actin (Santa Cruz, sc-47778) overnight at 4°C.

Techniques: Histone Deacetylase Assay, Binding Assay

Journal: The Journal of Neuroscience

Article Title: Dopamine Receptor Agonists Regulate Levels of the Serotonin 5-HT 2A Receptor and its mRNA in a Subpopulation of Rat Striatal Neurons

doi: 10.1523/JNEUROSCI.16-11-03727.1996

Figure Lengend Snippet: Average numbers (per 0.212 mm 2 of tissue section) of neurons labeled with the 5-HT 2A cRNA, double-labeled with the 5-HT 2A and PPE cRNAs, or Nissl-stained on adjacent sections in two sectors of the striatum

Article Snippet: The serotonin 5-HT 2A receptors were labeled with [ 125 I]DOI (DuPont, Billerica, MA; specific activity 2200 Ci/mmol), according to Mengod’s modification ( Mengod et al., 1990 ; Radja et al., 1993 ) of the protocol of McKenna et al. (1989) .

Techniques: Labeling, Control

Journal: Endocrinology, diabetes and metabolism journal

Article Title: Plasma Serotonin 2A Receptor Autoantibodies Predict Rapid, Substantial Decline in Neurocognitive Performance in Older Adult Veterans with TBI

doi: 10.31038/edmj.2022614

Figure Lengend Snippet: Mean two-year change in cognitive test performance on the St. Louis University Mental Status, Digit Symbol Substitution test, and Trails Making Test Part B according to baseline presence or absence of plasma autoantibody binding to serotonin 2A receptor peptide.

Article Snippet: Human Serotonin 2A Receptor Peptide An 18-meric linear synthetic peptide corresponding to the second extracellular loop of the human 5-HT2AR was synthesized at Lifetein, Inc (Hillsborough, NJ), catalog number 701781.

Techniques: Clinical Proteomics, Binding Assay

Journal: Endocrinology, diabetes and metabolism journal

Article Title: Plasma Serotonin 2A Receptor Autoantibodies Predict Rapid, Substantial Decline in Neurocognitive Performance in Older Adult Veterans with TBI

doi: 10.31038/edmj.2022614

Figure Lengend Snippet: Titer of plasma serotonin 2A receptor autoantibodies in representative patients with low or undetectable (‘antibody-negative’) vs. elevated baseline autoantibodies (‘antibody-positive’).

Article Snippet: Human Serotonin 2A Receptor Peptide An 18-meric linear synthetic peptide corresponding to the second extracellular loop of the human 5-HT2AR was synthesized at Lifetein, Inc (Hillsborough, NJ), catalog number 701781.

Techniques: Clinical Proteomics

Journal: Endocrinology, diabetes and metabolism journal

Article Title: Plasma Serotonin 2A Receptor Autoantibodies Predict Rapid, Substantial Decline in Neurocognitive Performance in Older Adult Veterans with TBI

doi: 10.31038/edmj.2022614

Figure Lengend Snippet: Change in Digit Symbol Substitution Test Performance in TBI patients having A) low, undetectable or B) present baseline plasma serotonin 2A receptor autoantibodies.

Article Snippet: Human Serotonin 2A Receptor Peptide An 18-meric linear synthetic peptide corresponding to the second extracellular loop of the human 5-HT2AR was synthesized at Lifetein, Inc (Hillsborough, NJ), catalog number 701781.

Techniques: Clinical Proteomics

Journal: Scientific Reports

Article Title: Early escitalopram administration as a preemptive treatment strategy against spasticity after contusive spinal cord injury in rats

doi: 10.1038/s41598-021-85961-5

Figure Lengend Snippet: Change of immunoreactivity of the 5-HT 2A receptor in the lumbar spinal cord motor neurons. ( A ) Representative microscopic images of immunoreactivity (IR) of the 5-HT 2A receptor (5-HT 2A R; red) on the lumbar motor neurons of each group are shown. As shown at the top, spinal motor neurons were stained by the anti-ChAT (green) antibody and the IR area of the 5-HT 2A R was confined to the ChAT-stained area (white lines in the binarized image indicate ChAT margins). The nuclei have been stained by 4′,6-diamidino-2-phenylindole (blue). Scale bar: 50 μm. ( B ) The bar graph with plots presents the quantitative analysis of the IR area of the 5-HT 2A R in the ChAT-positive area of lumbar motor neurons of each group (dashed bar with triangular plots: uninjured group, grey bar with round plots: vehicle-treated group, white bar with rectangular: escitalopram-treated group). *** P < 0.001.

Article Snippet: The primary antibodies we used were as follows: anti-choline acetyltransferase antibody (ChAT, 1:100, AB144P, Merck Millipore, USA), anti-serotonin 2A receptor antibody (1:200, PC176, Merck Millipore, USA) and anti-KCC2 antibody (1:1000, ab49917, abcam, UK).

Techniques: Staining